5CZR
Crystal Structure of Human Protocadherin-24 EC1-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97910 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.879, 119.769, 74.318 |
Unit cell angles | 90.00, 104.12, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.20617 |
Rwork | 0.205 |
R-free | 0.23546 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cyx |
RMSD bond length | 0.013 |
RMSD bond angle | 1.460 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.255 | |
Number of reflections | 50945 | |
<I/σ(I)> | 10.9 | 3 |
Completeness [%] | 95.5 | 80.3 |
Redundancy | 3.1 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 0.2 M MgCl 0.1 M HEPES pH 7.3 10% PEF 4000 |