5CZH
EGFR L858R MUTANT IN COMPLEX WITH AN OPTIMAL PEPTIDE SUBSTRATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 144.566, 144.566, 144.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.080 - 2.800 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.22000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2itv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.146 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.080 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.062 | 0.473 |
| Number of reflections | 12368 | |
| <I/σ(I)> | 16.6 | 2.2 |
| Completeness [%] | 98.8 | 99.3 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.1M HEPES 7.6, 0.15M NACL, 40% PEG400, 5MM TCEP, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
