5CZ2
Crystal structure of a two-domain fragment of MMTV integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.370, 83.150, 141.140 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 70.570 - 2.720 |
| R-factor | 0.243 |
| Rwork | 0.241 |
| R-free | 0.27990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5CZ1 and 3F9K |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.379 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.570 | 2.790 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.087 | 0.534 |
| Number of reflections | 33784 | |
| <I/σ(I)> | 9.5 | 2 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291.15 | 17% (w/v) PEG3350, 0.2M MgCl2, 4% (w/v) 1-Butyl-3-methylimidazolium dicyanamide |
