5CZ1
Crystal structure of the catalytic core domain of MMTV integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.886, 53.707, 69.647 |
| Unit cell angles | 69.69, 82.08, 63.97 |
Refinement procedure
| Resolution | 32.801 - 1.700 |
| R-factor | 0.1886 |
| Rwork | 0.187 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1asv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.954 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2084: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.620 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.571 |
| Number of reflections | 69082 | |
| <I/σ(I)> | 21 | 2 |
| Completeness [%] | 99.1 | 95.6 |
| Redundancy | 5.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 12.5% PEG3350, 0.15 M Ammonium Citrate Tribasic, pH6.5. |
