5CYR
Crystal structure of Thosea asigna virus RNA-dependent RNA polymerase (RdRP) complexed with ATP and ssRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2012-05-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 142.513, 158.339, 217.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.599 - 3.504 |
| R-factor | 0.2539 |
| Rwork | 0.252 |
| R-free | 0.29560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xhi |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.894 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 3.629 |
| High resolution limit [Å] | 3.504 | 3.504 |
| Rmerge | 0.310 | |
| Number of reflections | 27974 | |
| <I/σ(I)> | 5.57 | 2.3 |
| Completeness [%] | 89.6 | 75.92 |
| Redundancy | 8.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG 8000, Lithium sulfate |
