5CYO
High resolution Septin 9 GTPase domain in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.497, 78.049, 77.443 |
Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
Resolution | 55.292 - 2.035 |
R-factor | 0.1856 |
Rwork | 0.183 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.857 |
Data reduction software | MOSFLM |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.000 | 2.160 | |
High resolution limit [Å] | 2.035 | 6.070 | 2.035 |
Rmerge | 0.075 | 0.027 | 0.724 |
Rmeas | 0.091 | 0.033 | 0.875 |
Total number of observations | 131988 | ||
Number of reflections | 41927 | 1606 | 6631 |
<I/σ(I)> | 11.2 | 32.11 | 1.99 |
Completeness [%] | 99.0 | 97.2 | 97.4 |
Redundancy | 3.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG1500, 20% Glycerol |