5CYK
Structure of Ytm1 bound to the C-terminal domain of Erb1-R486E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 170.750, 170.750, 155.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 85.375 - 3.000 |
| R-factor | 0.2045 |
| Rwork | 0.202 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cxc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.345 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.380 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.176 | 1.862 |
| Number of reflections | 27367 | |
| <I/σ(I)> | 13.5 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.2 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.1M Hepes pH 7.5, 2M Ammonium Sulfate |
