5CY5
Crystal structure of the T33-51H designed self-assembling protein tetrahedron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 2 3 |
Unit cell lengths | 106.700, 106.700, 106.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 75.448 | 3.480 | |
High resolution limit [Å] | 3.400 | 15.190 | 3.400 |
Rmerge | 0.092 | 0.038 | 1.359 |
Rmeas | 0.094 | 0.039 | 1.388 |
Total number of observations | 154895 | ||
Number of reflections | 5827 | 81 | 428 |
<I/σ(I)> | 29.04 | 78.16 | 2.79 |
Completeness [%] | 99.9 | 98.8 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.8 | 298 | 100 mM HEPES pH 8.8 and 15 % PEG 3350 |