5CY2
Tn3 resolvase - site III complex crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2006-03-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.900, 151.920, 106.140 |
| Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
| Resolution | 49.247 - 4.000 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1000212338 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_2067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.070 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 19099 | |
| <I/σ(I)> | 22.9 | 1 |
| Completeness [%] | 99.5 | 96 |
| Redundancy | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | complex + well mixed 1:1 complex: 3mg/ml in 25mM tris pH 7.5 and 0.14M (NH4)2SO4 well: 16% PEG3350, 0.2M ammonium flouride cryoprotectant: added 20% ethylene glycol |
