5CY1
Tn3 resolvase - site III complex crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2009-03-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.313, 77.313, 272.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.427 - 3.400 |
| R-factor | 0.2506 |
| Rwork | 0.248 |
| R-free | 0.29190 |
| Structure solution method | MIR |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.538 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (dev_2067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 12184 | |
| <I/σ(I)> | 34.2 | 1.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | well + complex mixed 1:1 complex: 6mg/ml in 25mM tris pH 7.5, 0.18M (NH4)2SO4 well: 20% PEG3350, 0.2M sodium malonate pH 7.0 microseeding required cryoprotectant: crop conditions plus 20% PEG400 and 10% glycerol |
