5CXC
Structure of Ytm1 bound to the C-terminal domain of Erb1 in P 65 2 2 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 169.110, 169.110, 154.042 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.818 - 3.100 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4U7A and 3PSL |
RMSD bond length | 0.006 |
RMSD bond angle | 1.260 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.820 | 3.310 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.280 | 2.817 |
Number of reflections | 24180 | |
<I/σ(I)> | 17 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 39.1 | 40.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 294 | 15% PEG 4000,0.1M Na citrate pH 5.6, 0.2M Ammonium sulfate |