5CXB
Structure of Ytm1 bound to the C-terminal domain of Erb1 in P21 21 2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.443, 108.149, 108.618 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.533 - 2.100 |
| R-factor | 0.1648 |
| Rwork | 0.164 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cxc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.620 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.843 |
| Number of reflections | 60085 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 20% PEG 8000, 0.1M Hepes pH 7.5 |
