5CX3
Crystal structure of FYCO1 LIR in complex with LC3A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.426, 115.257, 73.517 |
| Unit cell angles | 90.00, 102.29, 90.00 |
Refinement procedure
| Resolution | 34.615 - 2.300 |
| R-factor | 0.1811 |
| Rwork | 0.178 |
| R-free | 0.24450 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.7.0029) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 25680 |
| <I/σ(I)> | 16.9 |
| Completeness [%] | 99.1 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 289 | 1.2 M sodium citrate tribasic dihydrate, 0.1 M HEPES sodium |
