5CWU

Crystal structure of Chaetomium thermophilum Nup188 TAIL domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-11-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97944
Spacegroup name	P 1
Unit cell lengths	65.276, 65.278, 155.548
Unit cell angles	90.00, 89.95, 90.01

Refinement procedure

Resolution	19.853 - 3.350
R-factor	0.2477
Rwork	0.246
R-free	0.27920
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.734
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.350
Number of reflections	33102
<I/σ(I)>	23.4
Completeness [%]	99.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	294	100 mM HEPES, pH 6.9 16 % (w/v) PEG 3,350 200 mM ammonium citrate