5CWT

Crystal structure of Chaetomium thermophilum Nup57

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	42.252, 42.361, 138.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.256 - 2.500
R-factor	0.2461
Rwork	0.241
R-free	0.29260
Structure solution method	AB INITIO PHASING
RMSD bond length	0.002
RMSD bond angle	0.443
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Number of reflections	8970
<I/σ(I)>	58.3
Completeness [%]	98.0
Redundancy	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	5 % (w/v) PEG 1,000 12 % (w/v) PEG 8,000