5CVS
GlgE isoform 1 from Streptomyces coelicolor E423A mutant soaked in maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9173 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.080, 114.080, 314.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.830 - 2.300 |
R-factor | 0.1821 |
Rwork | 0.181 |
R-free | 0.20910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cn6 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.442 |
Data reduction software | XDS |
Data scaling software | Aimless (0.3.6) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.830 | 62.830 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.069 | 0.030 | 1.026 |
Rpim | 0.016 | 0.008 | 0.245 |
Total number of observations | 1708887 | 19910 | 119311 |
Number of reflections | 92902 | ||
<I/σ(I)> | 27.6 | 81.2 | 3.5 |
Completeness [%] | 99.9 | 99.5 | 99.4 |
Redundancy | 18.4 | 16.1 | 17.9 |
CC(1/2) | 0.999 | 0.999 | 0.868 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | NULL |