5CVN
WDR48 (2-580):USP46~ubiquitin ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 103.437, 154.189, 182.854 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.360 - 3.360 |
| R-factor | 0.1772 |
| Rwork | 0.175 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 5CVL 1nbf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.170 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.360 | 50.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 7.210 | 3.350 |
| Rmerge | 0.045 | 0.719 | |
| Number of reflections | 21052 | ||
| Completeness [%] | 100 | 99.9 | |
| Redundancy | 7.3 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 292 | 0.1 M sodium chloride, 5% ethanol, 15% MPD, 0.1 M Tris pH 8.8 |
