5CVG
Crystal Structure of CK2alpha with a novel closed conformation of the aD loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.587, 44.086, 62.039 |
| Unit cell angles | 90.00, 110.38, 90.00 |
Refinement procedure
| Resolution | 35.130 - 1.250 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.16980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cvh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.4) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.130 | 1.254 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.042 | 0.446 |
| Rpim | 0.027 | 0.271 |
| Total number of observations | 231929 | 22648 |
| Number of reflections | 73445 | |
| <I/σ(I)> | 14.8 | 2.1 |
| Completeness [%] | 90.8 | 48.3 |
| Redundancy | 3.2 | 2.4 |
| CC(1/2) | 0.998 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 92mM Mes pH 6.5, 33% glycerol ethoxylate, 750mM ammonium acetate |
