5CV7
Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92D at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.114, 60.293, 38.737 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 38.640 - 1.650 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.843 |
| Data reduction software | APEX |
| Data scaling software | APEX |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.025 | 0.239 |
| Total number of observations | 165896 | |
| Number of reflections | 17257 | |
| <I/σ(I)> | 26.6 | 3.57 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.33 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
