5CV6
Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92E at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-07-03 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.084, 60.410, 38.623 |
Unit cell angles | 90.00, 93.64, 90.00 |
Refinement procedure
Resolution | 38.540 - 1.950 |
R-factor | 0.182 |
Rwork | 0.179 |
R-free | 0.23160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.806 |
Data reduction software | APEX |
Data scaling software | APEX (2013/1 for Windows) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.033 | 0.172 |
Total number of observations | 165896 | |
Number of reflections | 10473 | |
<I/σ(I)> | 23.56 | 5.34 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.91 | 10.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |