5CUP

Structure of Rhodopseudomonas palustris PduL - phosphate bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.098, 58.763, 136.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.227 - 2.100
R-factor	0.1977
Rwork	0.195
R-free	0.22840
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.713
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.230	2.210
High resolution limit [Å]	2.100	2.100
Rmerge		0.856
Number of reflections	27556
<I/σ(I)>	13.78	2
Completeness [%]	99.9	99.9
Redundancy	6.8	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	35% MPD