5CUL
crystal structure of the PscU C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-22 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 64 |
| Unit cell lengths | 68.570, 68.570, 61.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.660 - 2.900 |
| R-factor | 0.19701 |
| Rwork | 0.196 |
| R-free | 0.22452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jli |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.110 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.660 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.387 | 1.799 |
| Number of reflections | 3694 | |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1M di-ammonium hydrogen phosphate, 0.1 M sodium acetate, pH 4.5 |
