5CU6
Crystal Structure of CK2alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.527, 45.541, 63.596 |
| Unit cell angles | 90.00, 111.24, 90.00 |
Refinement procedure
| Resolution | 54.550 - 1.360 |
| R-factor | 0.1877 |
| Rwork | 0.187 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3war |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.4) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.550 | 1.364 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.055 | 0.543 |
| Rpim | 0.036 | 0.275 |
| Total number of observations | 220382 | 50520 |
| Number of reflections | 67061 | |
| <I/σ(I)> | 11.8 | 2 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 3.3 | 3 |
| CC(1/2) | 0.997 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 112.5mM Mes pH 6.5, 35% glycerol ethoxylate, 180 mM ammonium acetate |
