5CSL
Crystal structure of the 500 kD yeast acetyl-CoA carboxylase holoenzyme dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.100 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 159.866, 159.866, 614.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.930 - 3.200 |
R-factor | 0.22137 |
Rwork | 0.219 |
R-free | 0.26579 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | acetyl-CoA carboxylase unbiotinylated |
RMSD bond length | 0.011 |
RMSD bond angle | 1.532 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.148 | 0.036 | 0.901 |
Rmeas | 0.168 | 0.043 | |
Rpim | 0.099 | 0.024 | 0.633 |
Total number of observations | 389181 | ||
Number of reflections | 129365 | ||
<I/σ(I)> | 5.1 | ||
Completeness [%] | 97.5 | 98.9 | 87.4 |
Redundancy | 3 | 2.9 | 2.7 |
CC(1/2) | 0.998 | 0.448 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 14% (w/v) PEG3350, 4% (v/v) tert-butanol, and 0.2 M sodium citrate |