5CSJ
S100B-RSK1 crystal structure B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.960, 68.930, 91.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.115 - 2.700 |
| R-factor | 0.2067 |
| Rwork | 0.203 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3czt |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1750) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.120 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 7001 | |
| <I/σ(I)> | 9.19 | 2.19 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.3 | 13.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.1 M Hepes 7, 150 mM NaCl, 20% PEG6000 |
