5CRG
Human skeletal calsequestrin, D210G mutant high-calcium complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.179, 67.462, 158.062 |
| Unit cell angles | 90.00, 96.48, 90.00 |
Refinement procedure
| Resolution | 45.298 - 1.970 |
| R-factor | 0.1756 |
| Rwork | 0.175 |
| R-free | 0.19310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5crd |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9-1692) |
| Refinement software | PHENIX ((1.10-2152_1692: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.298 |
| High resolution limit [Å] | 1.970 |
| Number of reflections | 129904 |
| <I/σ(I)> | 10 |
| Completeness [%] | 96.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M MOPS, 27.5 % (v/v) 2-methyl-2,4-pentanediol, 0.2 M NaCl |
