5CQV
Crystal structure of uncharacterized protein Q8DWV2 from Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91730 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.940, 65.930, 55.750 |
| Unit cell angles | 90.00, 114.72, 90.00 |
Refinement procedure
| Resolution | 20.160 - 1.900 |
| R-factor | 0.1801 |
| Rwork | 0.178 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5com |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.160 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.439 |
| Number of reflections | 26530 | |
| <I/σ(I)> | 17.1 | 2.3 |
| Completeness [%] | 98.2 | 97.6 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.12 M Diethylene glycol, 0.12 M Triethylene glycol, 0.12 M Tetraethylene glycol, 0.12 M Pentaethylene glycol, 0.1 M Tris, 0.1 M Bicine, pH 8.5, 5% MPD, 12% PEG 550 MME, 6% PEG 20,000 |
