5CQK

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	50.630, 54.040, 66.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.918 - 1.880
R-factor	0.1751
Rwork	0.174
R-free	0.20320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cqd
RMSD bond length	0.009
RMSD bond angle	0.972
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((dev_2166: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	1.950
High resolution limit [Å]	1.880	1.880
Rmerge	0.207	0.168
Number of reflections	13173
<I/σ(I)>	10.4
Completeness [%]	98.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, MES-NaOH