5CQI

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	51.020, 54.290, 65.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.800 - 1.680
R-factor	0.1623
Rwork	0.160
R-free	0.19780
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.815
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2166: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.800
High resolution limit [Å]	1.680
Number of reflections	16702
<I/σ(I)>	17.9
Completeness [%]	95.0
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, MES-NaOH