5CQC
Crystal structure of the legionella pneumophila effector protein RavZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 221.778, 221.778, 72.536 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.942 - 2.985 |
R-factor | 0.218 |
Rwork | 0.214 |
R-free | 0.25040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.329 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 110.000 | 3.140 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.066 | |
Number of reflections | 18194 | |
<I/σ(I)> | 17.3 | 0.9 |
Completeness [%] | 99.2 | 98.2 |
Redundancy | 5.7 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 277.15 | 12% PEG 3350, 0.2 M BaCl2, 0.1 M MES |