5CP2
Human DNA polymerase lambda L431A mutant- Apoenzyme and complex with 6 paired DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | OTHER |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-06-30 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 83.876, 83.876, 396.363 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.950 - 2.360 |
R-factor | 0.209 |
Rwork | 0.206 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xsl |
RMSD bond length | 0.005 |
RMSD bond angle | 0.823 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.430 |
High resolution limit [Å] | 2.360 | 5.390 | 2.360 |
Rmerge | 0.066 | 0.026 | 0.529 |
Number of reflections | 35440 | ||
<I/σ(I)> | 13 | ||
Completeness [%] | 99.8 | 99.3 | 98.6 |
Redundancy | 13.7 | 12.2 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate |