5CMR

Crystal Structure of Linker-Mediated Zn-bound Human H-Ferritin variant 122H-delta C-star

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	I 4 3 2
Unit cell lengths	155.320, 155.320, 155.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.914 - 3.792
R-factor	0.2124
Rwork	0.207
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cei
RMSD bond length	0.005
RMSD bond angle	1.067
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	109.828	54.914	4.000
High resolution limit [Å]	3.792	11.990	3.790
Rmerge		0.032	0.555
Rmeas	0.194
Rpim	0.056	0.011	0.162
Total number of observations	38132	1226	5798
Number of reflections	3401
<I/σ(I)>	12	35	5
Completeness [%]	99.8	96.9	100
Redundancy	11.2	9.1	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	50 mM CHES, 150 mM sodium chloride, 0.3 mM zinc chloride, 1 mM H2BDH