5CMK
Crystal structure of the GluK2EM LBD dimer assembly complex with glutamate and LY466195
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 102.759, 102.759, 281.992 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.864 - 1.801 |
| R-factor | 0.1648 |
| Rwork | 0.164 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g3f |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.403 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.058 | 1.000 |
| Number of reflections | 82330 | |
| <I/σ(I)> | 48.4 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Reservoir: 2M Li2SO4 3% PEG 4K 0.1M MgSO4 0.1 M NaAcetate Protein buffer: 100 mM NaCl, 5 mM LY466195, 1 mM EDTA, 10 mM HEPES pH 7 No added glutamate |
