5CMC
Mnemiopsis leidyi ML032222a iGluR LBD E423S mutant glycine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.899, 123.138, 54.507 |
| Unit cell angles | 90.00, 111.72, 90.00 |
Refinement procedure
| Resolution | 39.109 - 1.280 |
| R-factor | 0.1355 |
| Rwork | 0.134 |
| R-free | 0.15680 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4yki |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1760) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.040 | 0.323 |
| Number of reflections | 144111 | |
| <I/σ(I)> | 34.92 | 3.5 |
| Completeness [%] | 96.6 | 81.9 |
| Redundancy | 4.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | Reservoir: 24% PEG 8K, 100 mM Cacodylate, 150 mM MgS04. Protein buffer: 150 mM NaCl, 10 mM HEPES, pH 7.0, 0.5 mM EDTA, 25 mM glycine |
