5CLW
Crystal structure of human glycogen branching enzyme (GBE1) in complex with maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 116.689, 164.536, 313.208 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 156.600 - 2.800 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bzy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 313.210 | 313.210 | 3.130 |
High resolution limit [Å] | 2.800 | 6.260 | 2.800 |
Rmerge | 0.153 | 0.052 | 0.758 |
Rpim | 0.028 | 0.384 | |
Total number of observations | 30030 | 97113 | |
Number of reflections | 74191 | ||
<I/σ(I)> | 9.2 | 22.7 | 2.1 |
Completeness [%] | 99.8 | 99.6 | 99.8 |
Redundancy | 4.5 | 4.3 | 4.7 |
CC(1/2) | 0.996 | 0.693 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 17% PEG 3350 , 0.2M sodium succinate |