5CKY
Crystal Structure of the MTERF1 R162A substitution bound to the termination sequence.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.217, 91.161, 159.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.550 - 2.620 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mva |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.689 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.550 | 2.624 |
| High resolution limit [Å] | 2.615 | 2.615 |
| Rmerge | 0.066 | 0.807 |
| Number of reflections | 19776 | |
| <I/σ(I)> | 25.4 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Sodium Acetate, 0.1M Tris HCl, 15.5% PEG4000 |
