5CKI
Crystal structure of 9DB1* deoxyribozyme (Cobalt hexammine soaked crystals)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.604 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.845, 80.845, 55.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 2.985 |
| R-factor | 0.2591 |
| Rwork | 0.256 |
| R-free | 0.31090 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.599 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.10pre_2086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.930 | 3.090 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.080 | 3.990 |
| Number of reflections | 4060 | |
| <I/σ(I)> | 25.23 | 0.86 |
| Completeness [%] | 100.0 | 98 |
| Redundancy | 4 | 24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | MPD, spermine, magnesium chloride, sodium chloride, sodium cacodylate |
