5CKG
Human beta-2 microglobulin mutant V85E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2014-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.578, 28.860, 87.719 |
| Unit cell angles | 90.00, 110.11, 90.00 |
Refinement procedure
| Resolution | 27.710 - 1.750 |
| R-factor | 0.188 |
| Rwork | 0.183 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z9t |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.789 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.710 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.064 | 0.320 |
| Number of reflections | 20777 | |
| <I/σ(I)> | 9.3 | 2.9 |
| Completeness [%] | 97.0 | 96.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M sodium acetate, 30% w/v PEG 4000, 15% glycerol, 0.2 M ammonium acetate |
