5CK9
E. coli MazF form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | P 61 |
Unit cell lengths | 52.190, 52.190, 197.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.110 - 1.898 |
R-factor | 0.1959 |
Rwork | 0.194 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ub4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.052 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.110 | 2.010 |
High resolution limit [Å] | 1.898 | 1.898 |
Number of reflections | 23960 | |
<I/σ(I)> | 17.68 | 1.64 |
Completeness [%] | 99.4 | 97 |
Redundancy | 11.2 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.1M Disodium Citrate pH 5.6, 0.2M Ammonium Acetate, 30%MPD |