5CK7
Mouse ADP-dependent Glucokinase; AMP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.681, 58.492, 161.708 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.640 - 2.990 |
| R-factor | 0.22817 |
| Rwork | 0.227 |
| R-free | 0.24315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ccf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.696 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.640 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.091 | 0.290 |
| Number of reflections | 9031 | |
| <I/σ(I)> | 10.1 | 2.6 |
| Completeness [%] | 97.1 | 80.2 |
| Redundancy | 5.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | Seeding of preequilibrated drops of 0.2 M NH4Cl, 20% w/v PEG 3350 |
