5CK4
Signal recognition particle receptor SRb-GDP from Chaetomium thermophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.99 |
Spacegroup name | P 1 |
Unit cell lengths | 34.960, 64.310, 64.930 |
Unit cell angles | 86.46, 90.13, 79.58 |
Refinement procedure
Resolution | 34.380 - 1.890 |
R-factor | 0.1671 |
Rwork | 0.165 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.046 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.380 |
High resolution limit [Å] | 1.890 |
Rmerge | 0.062 |
Number of reflections | 44352 |
<I/σ(I)> | 9.4 |
Completeness [%] | 97.7 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 0.2 M sodium acetate, 20% (w/v) PEG3350 |