5CJB

Human Osteoclast Associated Receptor (OSCAR) in complex with a collagen-like peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.940, 48.424, 118.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.438 - 2.398
R-factor	0.224
Rwork	0.222
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cj8
RMSD bond length	0.009
RMSD bond angle	1.232
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.398
Number of reflections	10822
<I/σ(I)>	18.2
Completeness [%]	99.7
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277.15	5% polyvinyl alcohol type II, 0.2M potassium acetate pH 7, 0.1M HEPES NaOH