5CIZ
E. coli RNA polymerase alpha subunit CTD in complex with CAP and DNA: A(5)-tract binding site for alpha CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 176.312, 176.312, 158.493 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.927 - 5.010 |
| R-factor | 0.1816 |
| Rwork | 0.178 |
| R-free | 0.21410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lb2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.660 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5.180 |
| High resolution limit [Å] | 5.010 | 5.010 |
| Rmerge | 0.051 | 0.733 |
| Number of reflections | 6646 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 18.7 | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 28% (w/v) PEG4000, 0.2 M ammonium acetate, 0.01 M sarcosine, 0.1 M sodium citrate, pH 5.6 |
