5CIP
Crystal Structure of Unbound 4E10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.660, 134.170, 81.860 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 42.970 - 2.480 |
| R-factor | 0.1854 |
| Rwork | 0.183 |
| R-free | 0.23119 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wy7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.268 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.610 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.077 | 0.215 |
| Number of reflections | 32268 | |
| <I/σ(I)> | 9.7 | 4.2 |
| Completeness [%] | 98.4 | 98.7 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES, 15% PEG 20,000 |
