5CHZ
Structure of wild-type human MBD4 bound to a G:T mismatch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.886, 54.875, 104.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.910 - 1.830 |
| R-factor | 0.17546 |
| Rwork | 0.174 |
| R-free | 0.20899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ofa |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.766 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.910 | 1.895 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.071 | 0.544 |
| Number of reflections | 20686 | |
| <I/σ(I)> | 30.7 | 3.15 |
| Completeness [%] | 97.7 | 96.8 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 21% ethylene glycol |
