5CHW

Structure of ISG15 in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-13
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	102.850, 103.960, 192.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.688 - 2.100
R-factor	0.1999
Rwork	0.198
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5chf
RMSD bond length	0.012
RMSD bond angle	1.398
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.198
Number of reflections	120936
<I/σ(I)>	15.1	1.37
Completeness [%]	99.7	100
Redundancy	54
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.7 M (NH4)2SO4, 0.06 mM ZnSO4