5CHN
Fab fragments of chikungunya virus neutralizing human monoclonal antibody 5M16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 41 |
| Unit cell lengths | 133.930, 133.930, 52.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.643 - 2.047 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3UJT and 4JY6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.037 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.643 | 2.140 |
| High resolution limit [Å] | 2.047 | 2.050 |
| Rmerge | 0.040 | 0.195 |
| Number of reflections | 57761 | |
| <I/σ(I)> | 28.4 | 6.9 |
| Completeness [%] | 97.2 | 81 |
| Redundancy | 7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG3350, Tris, ammonium formate |
