5CH4

Peptide-Bound State of Thermus thermophilus SecYEG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-18
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	97.829, 138.007, 115.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.393 - 3.640
R-factor	0.2567
Rwork	0.254
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zjs
RMSD bond length	0.002
RMSD bond angle	0.577
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.600
Number of reflections	9099
<I/σ(I)>	5.9
Completeness [%]	99.8
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.5	293	PEG 500DME, ZnSO4