5CGY
Fab fragment of Chikungunya virus neutralizing human monoclonal antibody 4J21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9787 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.224, 127.588, 83.912 |
Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.150 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.280 | |
Number of reflections | 55062 | |
<I/σ(I)> | 21.7 | 6.2 |
Completeness [%] | 94.2 | 98.5 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG2000, Tris, ammonium acetate |