5CFG
C2 crystal form of APE1 with Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.530, 45.120, 77.980 |
| Unit cell angles | 90.00, 105.15, 90.00 |
Refinement procedure
| Resolution | 41.390 - 1.800 |
| R-factor | 0.1857 |
| Rwork | 0.185 |
| R-free | 0.20410 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1bix |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.103 | |
| Number of reflections | 27028 | |
| <I/σ(I)> | 9.13 | 2.01 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20% PEG 8000, 0.2 M MgAc and 0.1 M Cacodylate |
